Pharma Tips

Centrifuge - Calibration and Operation

By: Pharma Tips | Views: 3597 | Date: 30-Aug-2011

To lay down an operating procedure and calibration of Centrifuge apparatus.

SOP FOR CALIBRATION AND OPERATION

1.0 OBJECTIVE

To lay down an operating procedure and calibration of Centrifuge apparatus.

2.0 SCOPE

This sop covers operation procedure and calibration for centrifuge apparatus and this sop is applicable to Quality control department.

3.0 RESPONSIBILITY

Officer / Executive.

4.0 ACCOUNTABILITY

Department Head

5.0 PROCEDURE



5.1 PROCEDURE FOR GENERAL CLEANING.

5.1.1 Clean the instrument with dry cloth from inside chamber and outside of the
         instrument.

5.1.2 Remove the glass pieces and solution immediately by removing centrifuge head of any breakage of centrifuge tubes observed.

5.1.3 Remove the centrifuge head once in a month, apply grease at threading and fix it properly.

5.2 PROCEDURE FOR OPERATION

5.2.1 Keep the instrument on stable platform.

5.2.2 Before operating the instrument, the speed knob and timer must be in ‘OFF’ position.

5.2.3 Put the main switch and the instrument switch to ‘ON’ position.


5.2.4 Place the equally filled tubes in the cavities of chamber and ensure the balance
        of centrifuge tubes by suitable selection of tube position.

5.2.5 Close the lid and set the desired time with timer knob. [The instrument can also be operated without using the timer.]

5.2.6 Slowly increase the speed with the speed knob to the required RPM.

5.2.7 After the time is completed slowly bring the speed knob to ‘OFF’ position, remove the tubes from the chambers, close the lid and 
        switch ‘OFF’ the instrument.

5.2.8 For vibration free performance:

5.2.8.1 Always keep the centrifuge tubes equally either side .

5.2.8.2 Use Rubber cushion for glass tubes.

5.2.9 In case, any discrepancy in instrument performance, inform to Utility department through department head for rectification.

5.3 CALIBRATION

5.3.1 RPM Calibration shall be done through utility department once a year and after each maintenance job.


6.0 REASON FOR REVIEW

First time issue.

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Comments
P siva Rama Krishna  |  22-Jun-2016 07:34:38 IST
Calibration for Hplc update
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